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1) MS/MS file
2) List file contains Mascot Generic file (MGF) files
3) MGFLIST (initial letter of the file name) is in the format of MGF
4) TXT files contains the corresponding MS/MS spectra
5) Convert MGFLIST.txt to MGF
6) Convert MGFLIST.txt to.dta

Good luck!

A single nucleotide polymorphism in TNF-alpha gene is associated with plaque progression in an apolipoprotein E-deficient mouse model of atherosclerosis.
Several studies have investigated the association between tumor necrosis factor (TNF)-alpha gene polymorphism and atherosclerosis development. We investigated the effects of single nucleotide polymorphism (SNP) in TNF-alpha gene on the development of atherosclerosis in the apolipoprotein (apo) E-deficient mice. We determined the plasma lipid and TNF-alpha levels, and examined the aortic lesions in apoE-deficient mice (C57BL/6J apoE-/-) with or without TNF-alpha (TNF) SNP. The animals received a high-fat diet. The aortic lesion area was measured by Oil Red O staining. The plasma lipid levels were analyzed with enzymatic methods. The TNF gene SNP was genotyped using the PCR-based method. The plasma TNF-alpha levels were significantly higher in TNF(+/1) apoE-/- than in TNF(1/1) apoE-/-, and the lesion area was significantly larger in TNF(+/+) apoE-/- than in TNF(+/1) apoE-/-. The plasma TNF-alpha levels and atherosclerotic lesion area were significantly larger in apoE-/- mice than in wild-type (WT) mice. The plasma TNF-alpha levels were not significantly different between apoE-/- mice with the TNF SNP and apoE-/- mice with WT TNF allele. We conclude that TNF-alpha SNP is associated with the development of atherosclerosis in apoE-/- mice. The increase in plasma TNF-alpha concentration in apoE-/- mice may play a role in the development of atherosclerosis.

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