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Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user personal data via analytics, ads, other embedded contents are termed as non-necessary cookies. It is mandatory to procure user consent prior to running these cookies on your website.Identification of proteins in bronchoalveolar lavage fluid.
The determination of the identity of proteins present in body fluids is a necessary task in clinical medicine. Advances in two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) have greatly facilitated this identification. However, the procedures so far developed require large amounts of very pure proteins in the test samples. The aim of this work was to develop a 2D-PAGE method that was able to detect proteins present in limited amounts in the bronchoalveolar lavage fluid (BALF). BALF was obtained from four healthy subjects. The proteins present in the BALF were then resolved on 2D gels prepared with either acrylamide/bisacrylamide in a ratio of 40/1 or bisacrylamide/toluene in a ratio of 1:1, and silver stained. The method developed allowed us to separate proteins of molecular weight between 14.3 and 80.5 kD