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Use of this software is permitted only if you accept all the terms set by the. Download Of Office 2013 2010 Cracked Dlll.Interaction of S-adenosylmethionine with DNA: novel insights based on chemical modification of DNA.
The major methylating agent in biological systems, S-adenosylmethionine (AdoMet), is a key enzyme in the bioactivation and bioinactivation of carcinogens, mutagens, and cytotoxic compounds. Since a proper understanding of the mechanism of AdoMet binding to DNA could provide significant information on the molecular basis of its biological actions, we decided to investigate the interactions of AdoMet with DNA. We chose an indirect assay based on the AdoMet-mediated hydrolysis of the oligonucleotide 5′-d[(p-nitrophenyl)thymidine 5′-d]3-. The value of this assay, which monitors both AdoMet binding and hydrolysis, allows us to determine quantitatively the rates of these two reactions. We show that AdoMet interacts rapidly with 5′-CG-3′ sites and slowly with 5′-TC-3′ sites. The binding to 5′-AC-3′ and 5′-CC-3′ sites is negligible. A significant enhancement in the rate of hydrolysis of oligonucleotides containing all 4 dC sites is observed. Treatment of DNA with NaIO4 leads to a large increase in the rates of both AdoMet binding and hydrolysis. Upon replacement of NaIO4 by NaIO4/H2O2, the rate of hydrolysis of AdoMet-treated DNA increases significantly,